FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238853
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14200.040.554638
GTCTTGC25300.034.3561361
GTCTCGC14200.032.6809771
CTGTCGC19600.030.8509659
TCGCTCT16050.029.601094
TAATACT8600.029.2960054
CGCTCTG14700.028.4020375
GTCTCAC30000.024.79951
TTGCTCT37550.024.7296854
CTCGCTC16900.024.2787883
TCTCGCT17800.024.2663022
GTACAGA13500.024.0083181
CTTGCTC32750.023.9581893
GGCTAGA14150.023.9466191
GTGTTAG19950.023.2616481
GGGTTTC43750.023.0666921
GTATTAC7500.022.5896431
CGTAACC1600.002268334422.4951463
GTGTTAC11850.022.3782941
TAGCACT14800.022.373554
Produced by FastQC (version 0.11.5)