FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109496
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.036.574268
GTCTCGC11600.033.1670761
GTCTTAC10600.032.8718871
GTCTTGC23650.031.001271
TCGCTCT12450.029.4888294
TCCGTCG1607.1707735E-526.9952898
GTCTCAC24950.026.7674241
TTGCTCT29250.026.0877554
GTGTAAA17750.025.3561081
CGCTCTG12650.025.0391085
TTACACT12100.024.3924354
CTGTCGC18200.024.1276579
CTCGCTC13450.024.0850143
TCTCGCT14700.024.008552
GTGTAAC7750.023.4168031
GGATAAC6950.022.9787881
GGGTTTC45850.022.64051
CTCGATC11800.022.5723328
AATGGCG17700.022.3689775
CGTTTCG1952.745105E-422.149989
Produced by FastQC (version 0.11.5)