FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109496
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14500.038.2274558
GTCTCGC13750.037.5036131
GTCTTGC24750.034.8247871
TCGCTCT15250.034.4586834
CGCTCTG14400.032.4935045
CTCGCTC15600.030.9168953
GTATTAA9800.029.3167861
CTGTCGC19700.029.2332069
TCTCGCT17650.028.551372
GTCTCAC27850.027.7742231
TTGCTCT31850.027.5737654
ATACCGA1607.171227E-526.9950396
GTGTTAC10000.026.5204161
TTACACT12900.025.6692854
GTTTCAC39900.025.4385223
TCTGTCA31650.024.7916538
TCACTCT33200.024.5011254
GGGTTTC44300.024.4450781
TAATACT7900.023.6914674
CTTGCTC30800.023.6056693
Produced by FastQC (version 0.11.5)