FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604726
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13850.039.503218
GTCTCGC14200.036.774051
TCGCTCT14950.034.6704834
GTCTTGC27500.031.9117091
TCTCGCT16350.029.5121142
CGCTCTG14900.028.505875
GTCTCAC31150.028.4052851
CTCGCTC17050.025.7557413
CTGTCGC23250.025.6994449
GTCTTAC10750.025.6373121
TTGCTCT39550.025.1189334
GTACTAA7300.024.837881
GAGTGCA78650.024.6219336
GGAGTGC72000.024.4963955
GTTTCAC42600.024.1654573
GGGTTTC46100.023.5986791
TTCACCG8600.023.438415
TCACTCT38900.023.3179054
GGCTAGA17550.023.1423951
TCTGTCA35100.022.9709788
Produced by FastQC (version 0.11.5)