FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387681
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.043.0692028
GTCTCGC13100.032.6729161
GTCTTGC24600.030.6694091
TCGCTCT13300.028.6865864
TCTCGCT14750.026.8529512
GTCTCAC33300.026.7958851
CTGTCGC18550.026.38889
CTCGCTC15000.025.4350643
GAGTGCA74200.023.2842356
GTGTAAA20400.023.1148131
GGAGTGC69150.023.006725
TTGCTCT35050.022.592244
GTCTCGA28700.022.5743486
CGCTCTG15350.022.510625
GGATAAT14650.022.283431
TCGAACT19400.022.264059
TCACTCT37200.022.2540744
GTGTTAC11500.022.0788971
GTCCTAG14550.021.937991
GGGTTTC46650.021.9267671
Produced by FastQC (version 0.11.5)