FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387681
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14100.038.666861
TCGCTCT13550.037.717374
GTCTTGC26750.035.805181
TCTGTCG15050.033.9581648
CTCGCTC16400.030.7239133
CGCTCTG15400.030.3819035
TCTCGCT17450.030.116262
CTTGCTC34200.026.9405353
CTGTCGC21600.026.327829
TTGCTCT35650.026.046694
GGGTTTC47850.025.0975861
TTCACCG9350.024.6354645
TCTGTCA34350.024.3082478
GTCTCAC30000.024.0675071
GTCTTAC9250.023.8949341
GGAGTGC66050.023.3218655
GGCTGGA89150.023.3052671
ACCGTGT9300.023.2199158
GTTTCAC44150.023.1515463
GTATAAA12450.023.0792451
Produced by FastQC (version 0.11.5)