FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275228
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.046.6480751
TCGCTCT13350.039.9020274
TCTGTCG11950.038.5528568
CTCGCTC14450.037.362673
TCTCGCT14850.036.861842
GTCTTGC22700.035.1630131
CGCTCTG15150.032.3102845
CTGTCGC18750.026.8745549
TTGCTCT33750.024.9549434
TCTGTCA31450.023.8043968
TTACACT12450.023.7060414
GTATTAA11400.023.5513671
TACACTG14800.023.346625
GGAGTGC63350.023.294415
GTTCTAA11250.023.2203771
GTTAGCC14950.023.1123733
CTTGCTC30550.023.0918853
GTCTCAC29050.022.7307871
CTATACT8100.022.2177184
GAGTGCA70700.022.1963526
Produced by FastQC (version 0.11.5)