FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275228
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC22200.037.1892741
GTCTCGC11750.033.2499241
TCTGTCG13300.027.0610228
TCGCTCT12800.026.4306034
GTATTAA9650.025.9719351
TTACACT14200.025.8524114
GGCTAGA14900.025.2311251
GGGTTTC40900.024.871871
CTCGCTC14350.024.5793293
CGCTCTG13050.024.269535
GGAGTGC65100.024.1043115
TTGCTCT33350.023.9577674
TCTCGCT15100.023.8373892
GAGTGCA68550.023.5212156
CTGGAGT73050.023.1565283
GGCTGGA80800.023.0813851
ACCCGTT3453.2287062E-922.9505356
CTTGCTC29300.022.847633
GTTTCAC37850.022.8213423
GTCTTAA9100.022.6813741
Produced by FastQC (version 0.11.5)