FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326412
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT13050.047.9921654
GTCTCGC14200.044.42871
TCTGTCG15000.042.7130288
TCTCGCT15600.038.3215982
CTCGCTC15450.036.8095253
CGCTCTG15350.034.2354555
GTCTTGC24950.033.1334761
GTCTTAC9850.030.1842441
CTGTCGC20300.030.1428629
GGGTTTC40000.028.4624541
TTGCTCT33000.027.7045674
TCTGTCA33850.025.0948688
CTTGCTC32350.024.25573
GTTTCAC38800.024.1197723
TTACTCT14800.023.8339484
GTTAGCC14200.023.8270973
TACCCGT2452.0359385E-623.5063655
GTCTCAC31550.023.4440981
GTGTTAG22700.023.320061
ATACCCG2502.4267447E-623.0362384
Produced by FastQC (version 0.11.5)