FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326412
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23000.045.1306341
GTCTCGC14350.043.6060941
TCGCTCT15750.041.591394
TCTGTCG16550.040.8857968
TCTCGCT17600.034.358592
CTCGCTC17450.033.8268053
CGCTCTG16800.033.4216545
TTGCTCT33850.030.1975864
GGTATAC5300.029.1691381
CTGTCGC23650.028.9162249
CTTGCTC32400.028.6607323
TCTTGCT35800.028.5544662
TAATACT8150.027.3808354
TTACACT13100.025.8267144
GTGTAAC8050.025.6060331
CTCGATC12250.025.2682388
ATGGCGT13700.025.2210546
AATGGCG15700.025.2177075
GTATAAA12500.024.7354281
TCTGTCA32250.024.5530578
Produced by FastQC (version 0.11.5)