FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.049.628918
GTCTCGC12600.048.5572171
GTCGTAC700.001033203841.6204721
CTGTCGC15200.041.204059
TCGCTCT14000.040.6243674
TCTCGCT15400.037.4363022
CTCGCTC15300.033.9104543
GTCTTGC26600.033.4059071
CGCTCTG16500.030.5422955
GGTATAC5600.029.9147151
GTGTTAG18700.027.6542431
GTGTAAC7500.027.192041
GTCTCAC34250.026.1570991
TAGTACT5400.025.3307154
GTCTTAC11500.024.7008441
GTATTAA9200.024.5425051
GTTAGCC15000.024.5003013
GTATTGG9400.024.0203251
TTGCTCT35200.023.1112444
GTATCGT1600.002118442422.7611941
Produced by FastQC (version 0.11.5)