FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13900.037.731241
TCGCTCT14300.034.2327164
TCTGTCG12800.033.1837968
GTCTTGC24500.030.451791
TCTCGCT16950.028.469252
CGCTCTG15950.027.9783235
CTCGCTC16800.026.9960213
GTACGAT1657.417677E-526.8611621
GTCTCAC32200.026.6109031
CTGTCGC16550.026.0998089
GTATTAT8250.025.9657921
GTATAAT8850.025.0400681
GTATTAC6350.024.4288541
GTATTAA7900.024.3110541
GTGTAAG12800.024.2380031
GTACTAC4900.024.120231
TGTACCG1801.5978822E-423.9921635
TTGCTCT34500.022.9531364
GTCTAAG9700.022.8458371
CGTTTCG1902.301549E-422.7343089
Produced by FastQC (version 0.11.5)