FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711069
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.046.5827678
CTGTCGC14550.039.0860339
GTCTCGC13150.038.241971
GGCGTAC1001.3497601E-436.4407161
GTCTTGC26350.034.8503271
TCGCTCT15000.034.0764
TCTCGCT15500.033.4727252
CTCGCTC15850.030.9121933
GTACGCG950.004874029730.3379733
CGCTCTG15550.029.6299635
TAGTCCG1753.9905135E-628.7966215
GTCTCAC33100.026.8626421
GTCTTAC11000.026.502341
TTGCTCT37050.025.843124
TCGATCT10900.025.0965849
GTGTTAG16650.024.95041
TCTCGAT10700.024.8942747
GTGTACG3851.4551915E-1124.6093141
GTATTAC7600.023.9741551
CTCGATC10950.023.6684558
Produced by FastQC (version 0.11.5)