FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667300
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC26150.038.971051
TCTGTCG10750.038.1707278
GTCTCGC10850.032.873971
CTGTCGC14350.031.1031119
TCGCTCT12450.028.332884
TTGCTCT35400.028.266734
GCGTAAA1409.766864E-425.997271
GTCTCAC32100.025.851491
GGGTTTC44950.025.4246941
GTCTTAC11850.025.1855431
GTATTAA6950.025.136931
TCTCGCT13850.024.9772262
CTTGCTC34250.024.8261283
GTATTAC6500.024.6374131
GTACTAC5350.024.4908891
GTGTTAG20100.024.264121
TTATACT8100.023.9962164
GTATAGA8650.023.5628431
GTACGGC1550.001767401423.4814071
CTCGCTC14700.023.0392383
Produced by FastQC (version 0.11.5)