FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694582
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.040.3683138
GTCTCGC12450.034.471471
GTCTTGC27200.033.9634631
CTGTCGC13450.033.719679
GTATTAT8100.028.7370431
GTATAGA7350.027.710721
TCTCGCT15650.027.6195352
TCGCTCT14700.026.9350914
TTGCTCT36200.026.2505554
GTGTTAC11750.026.0009121
GTCTCAC32500.025.515181
CTCGATC10450.025.4893388
TCGATCT10750.025.447219
GTATTAG8300.025.4154051
GGTATAC5750.025.3010941
CTCGCTC16150.024.979223
CGCTCTG15200.024.6282125
CTGTTGC29350.023.5465989
GTGTTAG16700.023.5209271
TCTCGAT11150.023.2434587
Produced by FastQC (version 0.11.5)