FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694582
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13200.037.6318328
GTCTTGC23800.035.956421
GTCTCGC13600.035.8014341
TCGCTCT13400.033.845414
TCTCGCT16450.029.7753582
CTGTCGC18200.029.2711989
CTCGCTC16050.028.7057573
CGCTCTG15650.028.5147045
GTATTAG8350.026.5051481
GTCTTAC10450.026.1204641
GTGTTAT10200.025.3141481
TTGCTCT35600.025.2769154
GGTATAC6550.024.7786031
TATAATA7000.024.6960322
GTATAAT7500.024.5908851
GTATTAC6350.024.3972571
ACCGTGT8500.023.714798
CTTGCTC36150.023.6975083
GTGTAAA14800.023.4278031
GTTAGCC13900.023.3056773
Produced by FastQC (version 0.11.5)