FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660705
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.045.1469888
GTCTTGC26800.035.5203861
CTGTCGC15700.034.3906759
GTCTCGC13500.033.8907131
GTATTAC5300.033.416281
TCGCTCT14200.029.9118144
TTGCTCT34700.029.2528864
GTATAAG7450.028.7252941
GTATTAA8000.028.595291
TCTCGCT16250.028.3694322
CTCGCTC15550.027.7779373
GTATTAT7000.026.3551031
GTCTCAC30250.025.8584961
CTTGCTC34600.025.5922283
GTCTTAC9750.024.976531
GGGTACG3001.41681085E-824.5980951
GTTTCAC41650.024.54443
GTATTAG7900.024.2867281
ACCGTGT8300.024.2861718
GTGTAAA14300.024.2540661
Produced by FastQC (version 0.11.5)