FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721460
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.042.681088
GTCTTGC25200.037.4506651
GTCTCGC11050.036.0314371
TCGCTCT12950.031.1302744
CTGTCGC17900.030.9672289
GTCTTAC10950.029.6270681
TTGCTCT35600.027.9057524
GTATTGG8000.027.6491221
GTCTACG2155.292404E-727.434791
TCTCGCT13700.027.3342442
GGTATAC5900.026.2432371
CTCGCTC13800.026.0828843
TTCACCG8050.025.9309795
CGCTCTG14550.025.230395
GTTTCAC38400.025.1210753
CTTGCTC35200.024.541623
GTATTCG1851.6341842E-423.9127561
GTATAAC5650.023.4895211
TCTGTCA35600.023.4570088
TCTCGAT11050.023.453357
Produced by FastQC (version 0.11.5)