FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894237
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13500.044.263518
GTCTCGC13750.042.9067231
TCGCTCT14000.038.568684
GTCTTGC26000.038.098561
CTGTCGC16700.036.640939
TCTCGCT15950.032.981972
GTATTCG1402.6631124E-531.2152081
CGCTCTG15550.030.0943185
CTCGCTC17850.029.4692463
GTGTTAG19550.028.314561
GTATAGG8450.026.720711
GTCTTAC12300.026.054971
GTATAGA8200.024.8706551
TTGCTCT39300.024.7310644
GTTAGCC17550.024.224753
GTCCTAC9350.024.1486641
TCTGTCA40400.024.057698
GTCTATA6450.023.7138771
CTTGCTC38400.023.644153
GTGTTAA12700.023.5138191
Produced by FastQC (version 0.11.5)