FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894237
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12450.039.3181578
GTCTCGC12950.034.8036421
GTCTTGC26750.032.86911
TCGCTCT14450.031.3853034
GTCTTAC11600.026.751981
CTGTCGC18100.026.6470939
TCTCGCT17400.026.4953442
GTATAGG7000.026.3880061
CGCTCTG15600.026.3010965
GTGTAAA17550.025.681321
GTGTTAG19100.025.5314311
GTATAAT7000.025.3324871
GTATTAC7450.024.7941021
CTCGCTC17900.024.1296943
TTGCTCT39600.023.995644
TAATACT8750.023.8585224
GTACTAG5650.023.5390381
GTGTACG3502.8176146E-923.2214451
GTATAAC5500.022.837621
GTACAGG16300.022.2112541
Produced by FastQC (version 0.11.5)