FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823197
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13850.048.3422168
GTCTCGC13450.046.0825961
TCGCTCT14150.042.735934
CTGTCGC18150.037.6825569
GTCTTGC26200.036.8935051
CTCGCTC16700.034.9177743
CGCTCTG16350.034.7753835
TCTCGCT17850.034.3008582
GGTATAC5700.031.0682161
GGGTACG2053.4665936E-728.7949311
GTCTTAC10350.028.516721
GTCCGAT1855.076621E-627.9194091
GTATTAA7950.027.8441561
GTTTCAC42100.027.0179883
TTGCTCT36400.026.3039254
GGGTTTC45900.026.2032321
GTGTTAG17100.025.8901791
GTATTGG7950.025.059741
GTCTCAC34050.024.9207251
GTATAAC4850.024.3421081
Produced by FastQC (version 0.11.5)