FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098240
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.031.1133758
CTGTCGC13150.027.9494829
GTCTCGC11900.027.0806181
TGTCGCC14400.025.76670610
GTCGCCC13200.025.19214811
TCGCCCA13800.024.35078412
TCGCTCT13650.023.5885814
GTCTTGC29500.022.0855711
GTATTAG8400.020.8501151
GTATATA9200.019.7985441
GCGTAGA2152.6120688E-919.5506171
TCTCGCT15250.019.5129472
GTATTAT6850.019.4316961
CGCTCTG14950.019.197085
TTGCTCT36100.018.5170924
CGTGTTA5850.017.9506810
CTCGCTC16250.017.4455073
GTTTCAC42300.016.8788053
GTGCGAC2108.523566E-716.6800921
TAATACT7400.016.5532384
Produced by FastQC (version 0.11.5)