FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494248
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.029.7235768
GTCTCGC9700.027.4426441
TGTCGCC10400.027.2626710
GTCGCCC10250.026.63751211
CTGTCGC10750.025.7238129
TCGCTCT11450.025.6753234
GTATTAC4050.025.0799711
TCGCCCA12450.023.05479212
GTATTAT5400.022.7016981
CGCTCTG12200.022.6635555
TACACCG1755.2641553E-921.999635
GAGTACG1957.748895E-1021.554141
CTCGCTC12850.020.971813
GCGTAAC850.003776337520.6032221
TCTCGCT12500.020.4446322
GTCTTGC22300.020.104311
GTACTAC3750.018.6802541
GTCTTAC10600.017.8431681
GTGTTAC8600.017.5127371
TTACACT10750.017.2547844
Produced by FastQC (version 0.11.5)