FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858102
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.027.1562238
CGCGAAT657.960955E-426.92411810
CTGTCGC13350.026.2182439
GTCTTGC27750.023.8560751
TGTCGCC13550.022.47319410
GTATTAT5900.021.372271
GTCGCCC12850.020.70118911
CGTGTTA5350.020.28115810
GTCTCGC12650.020.2130811
TCGCTCT13050.019.578544
GTATATA9450.019.2739941
GTATTAG7650.019.2303891
TCGCCCA14150.018.79931412
GTGTATA11500.018.2748411
GTATAGC4800.018.2431131
GTACTAT5850.017.962451
GTCTCAC32950.017.6462691
CGTTTCG1200.001341497117.5006779
TACGCGC1000.00969367517.5002236
TTGCTCT36450.017.0919024
Produced by FastQC (version 0.11.5)