FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775872
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.030.3649798
CTGTCGC12900.026.046249
TGTCGCC13300.024.73658610
GTCGCCC12700.023.7005411
GTATTAT5900.022.5466161
TCGCCCA13750.022.14522412
GTCTCGC12850.021.521561
GGTATAC4900.021.4326061
GTCTTGC24000.020.4205111
TCGCTCT13850.018.4489274
CGACCGG950.00721401218.42086210
CGTTTCG1150.001008669318.260689
GTATAAT5650.017.9679831
GTATTAA6450.017.9103491
TCCGTCG1200.001341931617.4998178
TCTCGCT14850.016.970872
GTATAAG6450.016.8248731
GTGTAGA11950.016.6977041
GTATTAG6300.016.6698061
GTATAGA6100.016.6424771
Produced by FastQC (version 0.11.5)