FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429607
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.028.7838548
GTCTCGC10350.026.0459211
CTGTCGC12300.025.894379
TGTCGCC12250.025.14328210
GTATAAT4600.023.5935481
TATATCG750.00183187923.337475
GTCGCCC11550.022.4246211
GTCTTGC23000.021.4625151
TCGCTCT11200.020.9412124
TCTCGCT12350.020.4084762
TCGCCCA14100.019.36202812
TAATACT6300.018.8922374
GTATTAG5800.018.1085071
GTATAGT4700.017.8773331
CTCGCTC13300.017.6347053
GTGTTAG14800.017.504891
GTATAGA5400.017.5048891
GTACTAC3450.017.2511961
GTATTAT4500.017.115891
GTCTCAC28950.017.0513951
Produced by FastQC (version 0.11.5)