FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429607
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.031.1497218
GTCTCGC10500.028.357061
TCGCTCT11100.027.1168734
TGTCGCC12200.025.8194410
CTGTCGC12400.025.4029989
GTCGCCC11150.023.5423911
CGCTCTG12900.021.9762275
TTATACT5650.021.6812214
CTCGCTC13450.021.5992643
TCTCGCT13550.021.44582
GTCTTGC23550.020.6754741
TCGCCCA12900.020.6199712
GTGTATA9850.020.270771
GTATTCG1055.4388324E-420.0167471
CGTGTTA3400.019.5586510
GATCGTA1107.465583E-419.0968632
GTGTTAG16150.018.8702771
TATACTG5850.018.5465725
GTATTAG7150.018.1270541
CGCCGTA1752.7997066E-617.9998468
Produced by FastQC (version 0.11.5)