FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408140
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.025.6997078
CTGTCGC12250.023.999989
GTCTTGC23500.023.092541
GTCGCCC11100.022.70268211
TGTCGCC12300.022.19510310
GTCTCGC10450.022.1123811
GTATAAG5100.021.281361
GTATAAT6100.020.662391
GTATAGG5200.020.1988091
TCGCCCA12750.019.76468812
TCGCTCT11800.019.579994
GTATTAG6100.019.5144791
GTATTAC4850.019.4908121
GTATAGA4800.018.964441
TACCCGT1702.118417E-618.532935
GTATATA7600.018.4269871
GTGTAGG10750.018.238431
GTGTATA7900.017.7272261
TCGCGTT1000.0096942117.49998510
GTCTCAC26600.016.979151
Produced by FastQC (version 0.11.5)