FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840815
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.021.8748428
GTATAGA3100.020.34321
GTACGGG1651.5794285E-619.110281
GTACTAA3700.018.9381161
CTGTCGC11000.018.454419
GTCTTGC19800.018.0485951
GTGTAAA8600.017.9251461
TGTCGCC11350.017.26920510
GTGTAAC5150.017.0075281
CGTATCA3104.9112714E-1116.93476565
GTATTAA4350.016.9136941
GTCGCCC10600.016.84009211
GTATAAG5050.016.650541
TCGCCCA12200.016.35291512
GTATAGT3651.8189894E-1216.317911
TCGCTCT11450.015.89484
GTCTAAG5750.015.8421441
GTCTCGC10450.015.7575991
GGTTAAT5250.015.3488911
CGCCGTA3201.3878889E-915.31184962
Produced by FastQC (version 0.11.5)