FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534372
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11700.026.3349131
TCTGTCG10400.025.5759058
TCGCTCT11350.023.7512764
CTGTCGC11850.023.6280869
TGTCGCC11450.022.61950710
GTCGTAT1102.916999E-522.2812961
TCTCGCT12300.021.3478622
GTCGCCC10750.020.511111
GTATTAG6150.020.4956871
GTCTTGC22850.020.0733661
GTACTAT4550.020.0076961
GTATAAC3150.020.0076941
TCGCCCA12150.018.72379912
CGCTCTG12800.018.599065
CTCGCTC13550.017.8279693
GTGTAGC9950.017.5947071
GTATATA6400.017.5067331
TATACTG6200.017.5055
GTATTAA5650.017.3518071
GGTATAC4050.017.2906021
Produced by FastQC (version 0.11.5)