FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534372
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.025.6429278
CTGTCGC11600.024.1373319
TGTCGCC10900.023.44011510
GTCTCGC10300.023.1215971
GTCTTGC23050.021.5756321
GTATAGG5500.021.013451
GTCGCCC11300.020.13252611
TCGCTCT11300.019.8222354
GGTATAC4950.019.810661
GTATTAG6800.019.571351
GGGTACG1852.2600216E-718.9310361
TCGCCCA13100.018.70235412
TTACACT12000.018.665944
GTGTATA10150.018.6326161
GTATTAA5650.018.5959721
CGTGTTA4150.018.55401810
GTGTTAG17000.017.7172221
GTGTTAC8800.017.511211
GTCTTAC8200.017.0841051
TCTCGCT12500.017.0834432
Produced by FastQC (version 0.11.5)