FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832617
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.030.2692898
CGTATCG755.5921628E-527.99945810
GTCTCGC12900.026.326251
CTGTCGC12600.025.555069
GTCTTGC24550.022.9604991
TGTCGCC13350.022.80854610
TCGCTCT13750.021.894774
TCTCGCT14750.021.3599782
GTCGCCC12500.020.99959211
GGTATAC4500.019.4506651
GTATAAG6850.019.422271
GTATTAT5600.019.3811991
CTCGCTC16200.019.01573
TCGCCCA15000.018.89988112
GTGTAAT9800.018.2201121
GTATTAG6450.017.9127061
GTATAAT5950.017.6527041
GTATCGA1200.001342017317.4996611
CGCTCTG16050.017.448565
GTGTTAG17950.017.1642651
Produced by FastQC (version 0.11.5)