FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832617
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.026.9236688
CTGTCGC12800.025.1571319
TGTCGCC13200.024.65995610
GTATACG1703.7325663E-922.6647071
GTCTTGC26600.022.6492161
GTCGCCC13300.021.57970211
GTCGTAG1355.9901868E-620.7569051
TCGCCCA14700.020.71555112
GTCTCGC11600.020.533231
GTGTTAG15950.020.2038211
GTATTAG7450.019.7469221
TAATAGT4350.018.5051884
TATACGG950.00720779918.423622
GTGTAAA12650.017.9982051
GTATAAG6250.017.9339661
GTCGTAT1200.001334855717.5136381
GTATAGA7100.017.2669661
GTCTTAC9950.017.2496131
TTGCTCT33500.017.2382874
AGCCGTA1653.3995326E-516.9694067
Produced by FastQC (version 0.11.5)