FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474273
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.028.67118
CTGTCGC10850.024.19439
GTCTCGC10200.024.038691
TGTCGCC11800.022.24645410
GTCTTGC22450.021.8436221
TCGCTCT11400.021.1833444
GTGTTAC9450.020.3865551
GTATTAA5700.020.2792571
GTGTATA10800.020.1085571
GTCGCCC11450.019.56420711
TCTCGCT12550.019.5272962
CTCGCTC13050.018.77483
GTATAGC4400.018.309991
TCGCCCA13000.017.77003912
GTGTAAG10550.017.5969091
GTGTTAG15150.017.5717051
GTGTAAC8450.017.410271
GTATAAA6950.017.1359061
GTATTAG7500.016.8133491
GTAGTCG1250.001764385616.79882829
Produced by FastQC (version 0.11.5)