FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576541
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.027.9989438
TGTCGCC11800.023.7285810
CTGTCGC11750.022.6380779
GTCGCCC10950.022.3742111
GTCTTGC19750.021.4482251
GTATAAT5750.020.7006591
GTATTAG7950.020.6968291
TCGCCCA12850.020.428112
GTCTCGC11650.020.1336251
GGTATAC3650.019.1827221
TCGCTCT11950.018.7466834
GTATTGG7350.018.5759221
TATAATA5350.018.3196262
GTGTTAC10200.018.1906741
GTATTAC4650.018.0688881
ATTATAC5950.017.648863
TAATACG1401.8656116E-417.5017854
GTATAAG6300.017.2263891
GTTACAC6750.017.1128583
GTATTAA4100.017.07731
Produced by FastQC (version 0.11.5)