FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576541
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.030.0015778
GTCGCCC10900.024.72676311
CTGTCGC11500.024.3491069
TGTCGCC11400.023.94896310
TCGCTCT12000.023.6255824
TCGCCCA12350.022.67420812
GTCTCGC11500.022.2390821
GTCTTGC24150.021.7603531
TCTCGCT12050.021.209772
GTATTAT5900.020.7829821
CGCTCTG13000.020.7315675
GCGTAGA1205.6792032E-520.43661
CTCGCTC13150.019.9627463
GTATAGA6600.019.6403681
GTCTTAC8900.018.5011921
TATAATA3800.018.4266592
GTATAAT6100.017.804251
CGTGTTA4550.017.69348510
GTATAAA8550.017.6195221
CGCCGTA2005.160491E-717.49920768
Produced by FastQC (version 0.11.5)