FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408865
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.026.634338
GTCTCGC10500.025.3494971
GTCTTGC22300.024.656981
CTGTCGC11300.023.850079
GTATTAT5100.021.9747981
TGTCGCC10750.021.81474310
TCTCGCT11700.021.2438262
GTGTATA10650.020.3885881
TCGCTCT10900.020.228614
GTATAGG5200.019.5316871
GTGTTAG15050.019.3146421
CTCGCTC12500.019.0395743
GTCGCCC10050.018.4586511
TAAGCGG1551.9868468E-518.0641125
GTGTTAC8200.017.9382691
GGGTTTC36400.017.8960271
CGTGTTA4000.017.50063510
TTGCTCT30900.017.216194
TTACACT10200.017.1562274
GTGTAAC7450.016.9235421
Produced by FastQC (version 0.11.5)