FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224263
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9000.026.832598
TCGCTCT10950.023.9749134
GTCTCGC10800.023.662991
CTGTCGC10100.023.5640689
TGTCGCC10200.023.3330510
GTACCGT1304.345302E-621.5405396
TCTCGCT11300.021.0639762
GTATATA7550.020.865851
GTCGCCC10100.020.79182411
CTCGCTC11650.020.7316113
CGCTCTG12400.020.6068255
TAATACT5600.020.0019284
GTCTTGC20900.019.9329321
GTATAGA5750.018.2651791
TCGCCCA12200.018.07355112
GTATTAT5450.017.9858951
GTATTAG7300.017.7439121
GGTATAC4550.017.6964841
GTGTAAC6850.017.3763621
GTGTTAC8100.017.288031
Produced by FastQC (version 0.11.5)