FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3638997
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10250.028.708981
TCGCTCT11700.026.0256564
CTGTCGC11200.024.3763549
TCTGTCG10400.024.2327828
TGTCGCC11800.023.13687910
TCTCGCT13800.021.8158042
CGCTCTG12900.020.8917735
TCGCCCA12500.020.44169812
GTCTTGC22350.020.062951
CTCGCTC14100.019.8587153
GTCGCCC12000.019.8349811
GTATTAG8200.018.370331
GTATAGA5950.018.2518581
ATAGTAC4450.018.0903953
GTATAGC5000.017.5158961
GTATAAC4250.017.3098261
GTATTGC6550.017.1147691
GTATACG1250.001752905316.815261
GTGTAGG11500.016.449711
GTATAAG6200.016.385841
Produced by FastQC (version 0.11.5)