FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326048
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.020.9338198
CTGTCGC10750.020.51159
TGTCGCC11400.018.42093810
GTATAGA4400.018.3005681
GTATTAT3900.017.9537351
GTCTCGC9900.017.6817071
GTATAAG6750.017.6345581
GTCGCCC10800.017.49989111
GCGCTAA1452.435865E-416.9012741
GTCCGAT1250.001760247216.8046951
TCGCTCT12150.016.7104784
CGTTTCG1503.1615398E-416.3332339
TAATACT4850.015.8788164
CGTCAGA4300.015.4694381
GTATTAC3653.45608E-1115.3467531
GTCTTGC20350.015.3114031
TCGCCCA13100.015.22891212
TTATACT4600.015.2198134
CGCTCTG12750.015.1004425
GTGTTAG14250.014.7409611
Produced by FastQC (version 0.11.5)