FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326048
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41490.12474263750853866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.025.327028
TGTCGCC11550.021.8191710
CTGTCGC11800.021.6535249
GTCTCGC11150.021.0510181
GTATACG1257.7761135E-519.618291
GTCGCCC11500.019.17535811
TCGCTCT12300.019.0644724
GTCTTGC21200.018.5078221
TCGCCCA12250.018.28709212
GTATTAC4300.017.9236891
GTATATA5150.017.6863941
GTGTTAC7200.017.516331
GTCGTAT1401.853749E-417.516331
GTACGTT1000.00968991117.5013211
CGCTCTG12350.017.0035425
TCTCGCT13400.016.9824662
GTATAGA4750.016.9631841
GTATTAG5800.016.912321
CGTATCA5000.016.80050870
GTGTATA7150.016.168921
Produced by FastQC (version 0.11.5)