FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862514
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA2350.023.8353061
GTATAAG5450.020.555221
GTCTTGC18100.018.1810051
TCTGTCG8350.018.0234038
GTATTAA3900.017.9528771
TGTCGCC9050.017.40400110
CTGTCGC9700.017.320279
GTCTCGC9450.017.0409831
GTATAAA5000.016.8038921
GTCTATA3401.0913936E-1116.4744031
GTATTAC3900.016.1575891
GTATAGA4550.016.1575871
GTGTAGA8550.015.968611
GTGTTAG12200.015.7823431
GTGTAAC4700.015.6419191
TCGCTCT9400.015.6400074
GTATTAG4750.015.4772681
TAATACT4600.015.2190544
GGTATAC2553.8966755E-715.1015371
GTGTAAA9100.015.0034741
Produced by FastQC (version 0.11.5)