FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862514
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42110.14710845082329727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2653.6379788E-1219.8295861
TCTGTCG8750.019.5995648
TGTCGCC9300.019.56945610
GTCTCGC9500.018.4380361
GTATTAA4400.018.3123231
TCGCTCT10050.018.10814
TATTAGA4100.017.9323832
GTGTAAC5150.017.6861931
CGTATCA4750.017.6859870
CTGTCGC9750.017.5893549
GTATTAG5600.016.8905581
GTATAAT3359.094947E-1216.731831
GTCGCCC10000.016.44992311
TACGACC1503.162996E-416.3321154
GTGTTAT5800.016.3081251
GTATAGG3451.4551915E-1116.246851
GTACTAA3301.2369128E-1015.92375851
CGCCGTA5600.015.62629167
GTCTTGC18300.015.5060841
CTATTAG4100.015.380021
Produced by FastQC (version 0.11.5)