FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3571658
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.028.5317928
TGTCGCC11250.023.95520410
CTGTCGC11300.023.849219
GTCTTAC8950.023.0803681
TCGCTCT11850.022.4519584
GTATTAG6600.022.28021
GTCTCGC11650.021.6381591
GTCTTGC22950.021.052811
GTCGCCC10450.020.76524711
GCGTAGT2302.8376235E-1019.7892461
GTATTAT6450.019.541441
CGCTCTG12750.019.4942785
GTATAGA6650.018.9537261
GTATATA7550.018.5492691
TACACCG2104.26935E-818.3371735
GTACTAC4100.017.9328441
TCGCCCA13000.017.7689712
GTACTAG3750.017.7392831
GTATAAT5600.017.505871
GTATAAC4000.017.505871
Produced by FastQC (version 0.11.5)