FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946969
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.028.8716588
GTCTCGC13250.027.2276121
TGTCGCC15200.026.02045210
TCGCTCT14000.025.2490984
CTGTCGC15050.024.6512179
GTCGCCC14750.023.49246811
TCTCGCT15400.021.8240362
GTCTTGC25950.021.5958771
TCGCCCA17850.021.17732212
CTCGCTC17050.019.9115623
GTCTTAC10350.019.6279931
CGCTCTG17050.019.2954965
TAATACT5850.019.1446134
GTATTGG9000.018.6804351
GTATAAG6050.018.526051
GTGTTAG17400.017.9155031
GTATTAA5500.017.8313221
GTATAGC4000.017.5129051
ATACCGT1602.6105397E-517.4995966
GTATAAT4650.017.3245961
Produced by FastQC (version 0.11.5)