FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527461
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.028.8463238
TGTCGCC12450.023.89766310
CTGTCGC12350.023.5243179
GTCGCCC11850.022.74491911
GTCTCGC10150.022.4310111
GTCTTGC24600.022.212171
GTCGTAC800.002656363921.8918021
GTATAGA5400.021.4053171
TCGCTCT11950.019.3300364
CGGTGTA4900.019.28575370
GGTATAC5200.018.8606321
TCGCCCA14800.018.6845912
GTATAAT5700.018.4352021
GTGTACG2104.242247E-818.3474161
CGCTCTG12600.018.333115
TTACACT10850.018.064044
GTGTTAG18250.017.4654621
GTGTAAT8650.017.4122091
GTATAAG6050.017.3687021
GTATAGG4850.017.3328911
Produced by FastQC (version 0.11.5)