FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427450
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9600.026.6147568
CTGTCGC11000.024.1823279
GTATTAG6800.023.69461
GTCTCGC11500.023.4527261
TGTCGCC10800.022.68566110
TCGCTCT11900.022.6472034
GTCTTGC21250.020.7688311
GTATAAT6200.020.3381421
GTATTAA6250.020.1754361
GTACTAG4050.019.8917621
TAATACT6350.019.8426484
GTATAAA7400.019.406741
GCGCGTT1107.4801996E-419.0913111
GTCGCCC10850.019.0326611
TAGTACG950.007213178618.4211714
CGCTCTG13700.018.3948155
GTATTAT5550.018.3022921
GTCTTAC9450.018.1620431
CTCGCTC13600.018.014563
TCTCGCT13900.017.8819872
Produced by FastQC (version 0.11.5)