FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607389
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.026.6184488
GTCTCGC12050.026.439071
TCGCTCT12200.025.8223764
TGTCGCC12450.023.33254410
TCTCGCT13900.022.6651882
CTGTCGC12950.022.4316719
GTCTTGC23000.022.2237241
CTCGCTC14300.021.2965283
TAATACT6450.020.0796224
CGCTCTG14250.019.6511825
GTCGCCC11950.019.62276611
GGTATAC5000.019.6055871
GTATTAT6500.018.8515241
TCGCCCA13400.018.28296312
GTATAAC3350.017.7662561
GTGTAAG11650.017.7303731
GTATATA7750.017.6179241
GTAATAC5000.017.502323
GTTAGCC13000.017.5023173
TTATACT6900.017.2481844
Produced by FastQC (version 0.11.5)