FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439851
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG400.00242022734.999639
TCTGTCG10400.025.9124348
CTGTCGC11500.023.4345349
GTCTCGC11950.022.8535821
GTATTAC4750.022.8504981
GTCTTGC21100.022.5675321
TCGCTCT12000.022.1719114
GTCGCCC11550.021.51492311
TGTCGCC12050.021.4935510
TCTCGCT13950.019.5745222
GTGTAAC7550.019.4773541
GTGTAAA12750.019.2227481
GTATTAT5500.019.0979251
GTATATA6850.018.9120581
GTATAAT5200.018.8530791
GGTATAC4100.018.7873881
GTATAAG6200.018.6358781
TCGCCCA14750.018.50827812
GTATAAA7000.018.50681
CTCGCTC13400.017.7653963
Produced by FastQC (version 0.11.5)