FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439851
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.027.858798
GTCTCGC10100.023.5852951
CTGTCGC11900.023.2370059
TGTCGCC12250.022.00194210
GTCTTGC22350.021.629941
TCGCTCT10100.020.7914964
GTCGCCC11650.020.43098811
TCGCCCA13000.019.65558212
GTATAAT5700.019.0520021
TCTCGCT12150.018.7272932
GTGTAAC5450.018.6404021
TATACGG1150.001007484618.263812
GTTTCAC32400.018.0390933
GGGTTTC35950.017.9296861
ACCGTGT7450.017.8534078
CGTGTTA4650.017.31335410
TTGCTCT29500.017.2029084
TTCACCG6600.016.9694675
GTATTAG7050.016.8944321
CTCGCTC12300.016.7876593
Produced by FastQC (version 0.11.5)